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Chapters
- 00:00Introduction
- 01:11Bacteria Thawing for Intratracheal Instillation
- 02:00Intratracheal Instillation of Live Bacteria
- 03:44Bronchoalveolar Lavage (BAL) and Lung Harvest
- 05:55Bronchoalveolar Lavage Processing
- 06:38Lung Processing for Single Cell Suspension
- 08:01Results: Experimental Model to Evaluate Resolution of Pneumonia
- 09:29Conclusion
This manuscript describes the establishment of an infectious model of pneumonia in mice and the respective characterization of injury resolution along with methods for growing bacteria and intratracheal instillation. A novel approach using high-dimensional flow cytometry to evaluate the immune landscape is also described.
