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Determining Binding Affinity (KD) of Radiolabeled Antibodies to Immobilized Antigens
 

Determining Binding Affinity (KD) of Radiolabeled Antibodies to Immobilized Antigens

Article DOI: 10.3791/63927-v • 07:39 min • June 23rd, 2022
June 23rd, 2022 •
1, 1, 1, 1
1Department of Radiology and Biomedical Imaging, Yale University

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요약
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Here, a method is described to determine the binding affinity (KD) of radiolabeled antibodies to immobilized antigens. KD is the equilibrium dissociation constant that can be determined from a saturation binding experiment by measuring the total, specific, and nonspecific binding of a radiolabeled antibody at various concentrations to its antigen.

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Keywords: Binding Affinity KD Radiolabeled Antibodies Immobilized Antigens Nuclear Imaging Nuclear Therapy Antigen Immobilization Binding Buffer Blocking Buffer Washing Buffer BSA Tween 20 Serial Dilutions
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