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使用差分动力学显微镜定量细胞骨架动力学
JoVE 신문
생체공학
This content is Free Access.
JoVE 신문 생체공학
Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy
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使用差分动力学显微镜定量细胞骨架动力学

DOI:
10.3791/63931-v

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06:37 min

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June 15, 2022

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, , , , , ,
1Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science,Utrecht University, 2Department of Physics and Biophysics,University of San Diego, 3Department of Bionanoscience, Kavli Institute of Nanoscience Delft,Delft University of Technology

Chapters

  • 00:04Introduction
  • 00:45Image Acquisition and Parameter Setup
  • 01:55Calculating the DDM Matrix
  • 02:54Fitting the DDM Matrix or the ISF
  • 03:38Interpreting the Fit Results
  • 04:26Results: DDM Analysis for Vimentin and Actin-Microtubule Composite Network
  • 06:09Conclusion

Summary

자동 번역

자동 번역

差分动态显微镜（DDM）结合了动态光散射和显微镜的特征。本文介绍了利用DDM通过量化维门汀网络中颗粒的亚扩散和笼中动力学以及活性肌球蛋白驱动的肌动蛋白-微管复合物的弹道运动来表征重构细胞骨架网络的过程。

Tags

Keywords: Cytoskeleton DynamicsDifferential Dynamic MicroscopyReconstituted Cytoskeleton NetworksSoft MaterialsBiologically Relevant MaterialsMicroscope Control SoftwarePyDDM Code RepositoryDDM MatrixIntermediate Scattering FunctionData AnalysisXarray PackageBackground Estimation

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