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Kvantificering af cytoskeletdynamik ved hjælp af differentiel dynamisk mikroskopi
JoVE 신문
생체공학
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JoVE 신문 생체공학
Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy

Kvantificering af cytoskeletdynamik ved hjælp af differentiel dynamisk mikroskopi

DOI:
10.3791/63931-v

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06:37 min

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June 15, 2022

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, , , , , ,
1Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science,Utrecht University, 2Department of Physics and Biophysics,University of San Diego, 3Department of Bionanoscience, Kavli Institute of Nanoscience Delft,Delft University of Technology

Chapters

  • 00:04Introduction
  • 00:45Image Acquisition and Parameter Setup
  • 01:55Calculating the DDM Matrix
  • 02:54Fitting the DDM Matrix or the ISF
  • 03:38Interpreting the Fit Results
  • 04:26Results: DDM Analysis for Vimentin and Actin-Microtubule Composite Network
  • 06:09Conclusion

Summary

자동 번역

자동 번역

Differentiel dynamisk mikroskopi (DDM) kombinerer funktioner i dynamisk lysspredning og mikroskopi. Her præsenteres processen med at bruge DDM til at karakterisere rekonstituerede cytoskeletnetværk ved at kvantificere den subdiffusive og burede dynamik af partikler i vimentinnetværk og den ballistiske bevægelse af aktive myosindrevne actin-mikrotubululkompositter.

Tags

Keywords: Cytoskeleton DynamicsDifferential Dynamic MicroscopyReconstituted Cytoskeleton NetworksSoft MaterialsBiologically Relevant MaterialsMicroscope Control SoftwarePyDDM Code RepositoryDDM MatrixIntermediate Scattering FunctionData AnalysisXarray PackageBackground Estimation

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