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1Laboratory of Preclinical Research, Department of Research and Innovation,Groupe Hospitalier Paris Saint Joseph, 2Inserm UMR S 999, Pulmonary Hypertension: Pathophysiology and Novel Therapies, Marie Lannelongue Hospital,Groupe Hospitalier Paris Saint Joseph, 3Department of Cardiac Surgery,Marie Lannelongue Hospital
Chapters
- 00:05Introduction
- 01:02Heart Procurement and Instrumentation for Normothermic Ex Situ Heart Perfusion
- 03:06Connection to the NESP Machine and Resuscitation of the Heart
- 04:15Working Mode Procedure
- 04:54Pressure-Volume (PV) Loop Assessment with the Conductance Method
- 05:48Epicardial Echocardiography Assessment of the Heart in a Working State
- 06:42Results: Resuscitation of the Heart During Normothermic Ex Situ Heart Perfusion
- 07:39Conclusion
A reliable noninvasive approach for functional assessment of the donor heart during normothermic ex situ heart perfusion (NESP) is lacking. We herein describe a protocol for ex situ assessment of myocardial performance using the epicardial echocardiography and conductance catheter method.
