<em>In Vitro</em> Assay of <em>Plasmodium</em>-Infected Red Blood Cell Killing by Cytotoxic Lymphocytes

00:04Introduction
00:58Experimental Malaria Infection in Mouse
01:49Obtaining Fresh Whole Mouse Splenocytes
03:30Cytotoxic Effector Cell Purification: CD8a⁺ T Cell Negative Selection
04:22P. yoelii Infected RBC Isolation
05:21CFSE Labeling of RBCs, Preparation for Flow Cytometry and Cytotoxic Lymphocyte Coculture
06:22Results: P. yoelii – iRBC Enrichment and Assessment of cell lysis Percentage of P. yoelii – iRBCs by CD8⁺ lymphocytes
07:39Conclusion

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Here we describe a new method to help elucidate the mechanisms of cellular immunity to Plasmodium during the blood stage of infection. This is an in vitro assay that measures infected red blood cell killing by cytotoxic lymphocytes.

In Vitro Assay of Plasmodium-Infected Red Blood Cell Killing by Cytotoxic Lymphocytes

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