/
/
In vitro Ensaio de Morte de Hemácias Infectadas por Plasmodium por Linfócitos Citotóxicos
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:04Introduction
- 00:58Experimental Malaria Infection in Mouse
- 01:49Obtaining Fresh Whole Mouse Splenocytes
- 03:30Cytotoxic Effector Cell Purification: CD8a+ T Cell Negative Selection
- 04:22P. yoelii Infected RBC Isolation
- 05:21CFSE Labeling of RBCs, Preparation for Flow Cytometry and Cytotoxic Lymphocyte Coculture
- 06:22Results: P. yoelii – iRBC Enrichment and Assessment of cell lysis Percentage of P. yoelii – iRBCs by CD8+ lymphocytes
- 07:39Conclusion
