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In vitro Ensayo de destrucción de glóbulos rojos infectados con plasmodio por linfocitos citotóxicos
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Chapters
- 00:04Introduction
- 00:58Experimental Malaria Infection in Mouse
- 01:49Obtaining Fresh Whole Mouse Splenocytes
- 03:30Cytotoxic Effector Cell Purification: CD8a+ T Cell Negative Selection
- 04:22P. yoelii Infected RBC Isolation
- 05:21CFSE Labeling of RBCs, Preparation for Flow Cytometry and Cytotoxic Lymphocyte Coculture
- 06:22Results: P. yoelii – iRBC Enrichment and Assessment of cell lysis Percentage of P. yoelii – iRBCs by CD8+ lymphocytes
- 07:39Conclusion
