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JoVE Journal Cancer Research
Positron Emission Tomography Imaging of Cell Trafficking
 

Positron Emission Tomography Imaging of Cell Trafficking: A Method of Cell Radiolabeling

Article DOI: 10.3791/64117-v • 10:07 min • October 27th, 2023
October 27th, 2023 •
1, 2, 1
1Division of Nuclear Medicine, Department of Radiology, Mayo Clinic, 2Department of Radiology, University of Colorado Anschutz Medical Campus

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요약
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Presented here is a protocol to radiolabel cells with a positron emission tomography (PET) radioisotope, 89Zr (t1/2 78.4 h), using a ready-to-use radiolabeling synthon, [89Zr]Zr-p-isothiocyanatobenzyl-desferrioxamine ([89Zr]Zr-DBN). Radiolabeling cells with [89Zr]Zr-DBN allows noninvasive tracking and imaging of administered radiolabeled cells in the body with PET for up to 7 days post-administration.

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Keywords: Positron Emission Tomography Cell Trafficking Cell Radiolabeling Zirconium-89 DBN Non-invasive Tracking PET Imaging Cell Therapies Neurological Diseases Cancers Radiolabeling Technique Hydroxamate Resin Hydrochloric Acid Yttrium Dipotassium Phosphate HEPES-KOH Potassium Carbonate DFO-Bn-NCS Oxalic Acid Zirconium-89 Chloride
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