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秀丽隐杆线虫肠的手部解剖
JoVE 신문
생물학
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JoVE 신문 생물학
Hand Dissection of Caenorhabditis elegans Intestines
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秀丽隐杆线虫肠的手部解剖

DOI:
10.3791/64120-v

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05:41 min

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September 13, 2022

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, , ,
1Department of Biochemistry & Molecular Biology,Colorado State University

Chapters

  • 00:05Introduction
  • 00:31Preparation of Stock Solutions and Microcapillary Pipettes
  • 01:10Experimental Preparation
  • 01:56Hand Dissection of the Caenorhabditis elegans Intestine
  • 03:39Results: Isolating Intestines from Adult Caenorhabditis elegans Nematode Worms
  • 05:14Conclusion

Summary

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자동 번역

本协议描述了一种手动从 秀丽隐杆线 虫成虫中分离肠道的程序，以输入基因组学、蛋白质组学、微生物组或其他测定。

Tags

Keywords: Hand DissectionCaenorhabditis ElegansIntestinesPost BiologyMicro Capillary PipettesM9 BathDissection BufferBovine Serum AlbuminLevamisoleNematode Growth MediumHypodermic NeedleChelation Buffer

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