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JoVE Journal
Immunology and Infection
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Identifying Caspases and their Motifs that Cleave Proteins During Influenza A Virus Infection
 

Identifying Caspases and their Motifs that Cleave Proteins During Influenza A Virus Infection

Article DOI: 10.3791/64189-v 08:16 min July 21st, 2022
July 21st, 2022

챕터

요약

Influenza A virus (IAV) infection activates the caspases that cleave host and viral proteins, which, in turn, have pro- and antiviral functions. By employing inhibitors, RNA interference, site-directed mutagenesis, and western blotting and RT-qPCR techniques, caspases in infected mammalian cells that cleave host cortactin and histone deacetylases were identified.

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Keywords: Caspases Caspase Cleavage Influenza A Virus Infection Protein Degradation MDCK Cells A549 Cells Western Blotting SiRNA Transfection Cell Lysis SDS-PAGE
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