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JoVE Journal
Developmental Biology

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En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos
 

En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos

Article DOI: 10.3791/64207-v 08:57 min July 27th, 2022
July 27th, 2022

챕터

요약

Classically, the endocardium of the mouse embryonic valve primordium has been analyzed using transversal, coronal, or sagittal sections. Our novel approach for en face, two-dimensional imaging of the endocardium at valvulogenic regions allows planar polarity and cell rearrangement analysis of the endocardium during valve development.

Tags

Keywords: En Face Endocardial Cushion Planar Morphogenesis Mouse Embryos Primitive Endocardium Valve Development Planar Cell Polarity Cardio Development Genotyping Fixation Immunostaining
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