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小鼠骨髓造血干细胞和祖细胞以及基质生态位细胞的流式细胞术分析

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Flow Cytometry Analysis of Murine Bone Marrow Hematopoietic Stem and Progenitor Cells and Stromal Niche Cells

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小鼠骨髓造血干细胞和祖细胞以及基质生态位细胞的流式细胞术分析

DOI:

10.3791/64248-v

•

08:34 min

•

September 28, 2022

•

Laura Mosteo, Joana Reis, Lídia Rocha, Marta Lopes, Delfim Duarte^2,3,4

¹Instituto de Investigação e Inovação em Saúde (i3S),Universidade do Porto, ²Department of Onco-Hematology,Instituto Português de Oncologia (IPO)-Porto, ³Unit of Biochemistry, Department of Biomedicine,Faculdade de Medicina da Universidade do Porto, ⁴Porto Comprehensive Cancer Center (P.CCC)

Chapters

00:04Introduction
00:43Sample Extraction
01:47Sample Processing for Analysis of Hematopoietic Cell Populations in Total Bone Marrow
03:19Sample Processing for the Analysis of Stromal Cell Populations in Total Bone Marrow
05:13Sample Processing for the Analysis of Hematopoietic Cell Populations in Crushed and Flushed Bone Marrow
06:28Results: Phenotypic Analysis of Murine Bone Marrow Hematopoietic Stem and Progenitor Cells and Stromal Niche Cells by Flow Cytometry
07:40Conclusion

Summary

자동 번역

在这里，我们描述了一种简单的方案，用于将小鼠骨髓细胞分离和染色为表型造血干细胞和祖细胞以及支持生态位内皮和间充质干细胞。还包括富集位于内膜和中央骨髓区域的细胞的方法。

Tags

Keywords: Flow Cytometry Murine Bone Marrow Hematopoietic Stem Cells Hematopoietic Progenitor Cells Stromal Cells Niche Endosteal Central Bone Marrow RBC Lysis Cell Staining

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