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Chapters
- 00:05Introduction
- 00:45Processing the Ovarian Tissue and Isolation of Antral Follicles
- 01:50Isolation of Follicle Resident Cells
- 03:01Fluorescence-Activated Cell Sorting
- 03:36Processing of the Ovarian Cortical Tissue and Ovarian Tissue Slow Freezing
- 04:24Results: Identification and Purification of Ovarian Cells from Antral Follicles
- 05:45Conclusion
Here, we present protocols for the identification and purification of ovarian cells from antral follicles. We elaborate on methods for processing whole ovaries for the cryopreservation of cortical strips while also harvesting intact antral follicles that are treated enzymatically to liberate multiple follicle resident cell types, including granulosa, theca, endothelial, hematopoietic, and stromal cells.
