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Journal / Developmental Biology / 从健康和视网膜疾病特异性人诱导的多能干细胞中生成视网膜类器官…
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Developmental Biology
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JoVE Journal Developmental Biology
从健康和视网膜疾病特异性人诱导的多能干细胞中生成视网膜类器官
 
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从健康和视网膜疾病特异性人诱导的多能干细胞中生成视网膜类器官

Article DOI: 10.3791/64509-v • 09:47 min • December 9th, 2022
December 9th, 2022 •
*1,2, *1,2, *1,2, 1, 1,3, 1
1Centre for Ocular Regeneration, Prof. Brien Holden Eye Research Centre, LV Prasad Eye Institute, 2Manipal Academy of Higher Education, 3Schepens Eye Research Institute, Massachusetts Eye and Ear, Harvard Medical School
* These authors contributed equally

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该协议描述了一种将hiPSCs分化为眼场簇并使用涉及贴壁和悬浮培养系统的简化培养条件生成神经视网膜类器官的有效方法。其他眼细胞类型，如RPE和角膜上皮，也可以在视网膜培养物中从成熟的眼视野中分离出来。

Tags

发育生物学，第190期，干细胞，iPSC，视网膜分化，眼野原基，视网膜类器官，视网膜色素上皮
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