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从植物叶片组织和蓝藻中分离出塑体球脂滴
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Plastoglobule Lipid Droplet Isolation from Plant Leaf Tissue and Cyanobacteria
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从植物叶片组织和蓝藻中分离出塑体球脂滴

DOI:
10.3791/64515-v

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10:35 min

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October 06, 2022

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, , ,
1Department of Biochemistry and Molecular Biology,Michigan State University, 2Plant Resilience Institute,Michigan State University

Chapters

  • 00:04Introduction
  • 00:57Crude Plastoglobule Extraction from Un-Stressed Maize Leaf Tissue
  • 04:41Crude Plastoglobule Extraction from Cyanobacteria
  • 07:06Harvesting Pure Plastoglobules
  • 07:55Cyanobacteria Processing
  • 08:43Results: Isolation of Plastoglobule Lipid Droplets Associated with Various Photosynthetic Organisms
  • 09:36Conclusion

Summary

자동 번역

자동 번역

提出了一种快速有效的方案，用于分离与各种光合生物相关的质球脂滴。成功制备分离的质体球是蛋白质组学和脂质组学分析等详细分子研究之前的关键第一步。

Tags

PlastoglobuleLipid DropletIsolationCyanobacteriaThylakoidSucrose GradientUltracentrifugationSonicationMaizeLeaf FiltrateCentrifugationResuspensionBiochemical Analysis

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