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Chapters
- 00:00Introduction
- 00:59Tissue Extraction
- 01:36Tissue Digestion and Processing
- 02:43Results: A Two-Phase Differentiation Procedure
- 04:15Conclusion
Preadipocytes are isolated from the stromal vascular fraction of interscapular brown adipose tissue from newborn mice and differentiated into cells that accumulate lipid droplets, express molecular markers, and show the mitochondrial morphology of mature brown adipocytes. These cells are further analyzed by immunofluorescence and transmission electron microscopy.
