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JoVE Journal
Bioengineering

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Reconstituting Cytoarchitecture and Function of Human Epithelial Tissues on an Open-Top Organ-Chip
 

Reconstituting Cytoarchitecture and Function of Human Epithelial Tissues on an Open-Top Organ-Chip

Article DOI: 10.3791/64633-v 09:46 min February 17th, 2023
February 17th, 2023

챕터

요약

The present protocol describes the capabilities and the essential culture modalities of the Open-Top Organ-Chip for the successful establishment and maturation of full-thickness organ-on-chip cultures of primary tissues (skin, alveolus, airway, and intestine), providing the opportunity to investigate different functional aspects of the human epithelial/mesenchymal and vascular niche interface in vitro.

Tags

Keywords: Open-top Organ-chip Epithelial Tissue Microfluidic Microenvironment Mesenchymal Cells Collagen Hydrogel Surface Micropatterning Tissue-specific Function
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