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Un innovador inserto impreso en 3D diseñado para permitir cultivos celulares sencillos en 2D y 3D
JoVE 신문
생물학
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JoVE 신문 생물학
An Innovative 3D-Printed Insert Designed to Enable Straightforward 2D and 3D Cell Cultures
DOI:

08:17 min

January 06, 2023

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Chapters

  • 00:04Introduction
  • 00:57Placement of the Sterilized Insert in the 6‐Well Plates
  • 01:59Seeding of the Cells
  • 04:21Implementation of the Co‐Culture, Cell Observation, Counting, and Scraping
  • 05:12Insert Cleaning for Recycling
  • 05:41Results: Paracrine Intercellular Communication Between Endothelial Cells and Keratinocytes
  • 07:38Conclusion

Summary

자동 번역

En este trabajo, se presenta un inserto impreso en 3D de nuevo diseño como modelo de cocultivo y se valida mediante el estudio de la comunicación intercelular paracrina entre las células endoteliales y los queratinocitos.

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