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Malachite Green Assay for the Discovery of Heat-Shock Protein 90 Inhibitors
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Malachite Green Assay for the Discovery of Heat-Shock Protein 90 Inhibitors

Malachite Green Assay for the Discovery of Heat-Shock Protein 90 Inhibitors

DOI:
10.3791/64693-v

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07:57 min

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January 20, 2023

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, , , , , ,
1Gangneung Institute of Natural Products,Korea Institute of Science and Technology (KIST), 2School of Life Sciences,University of Sussex

Chapters

  • 00:04Introduction
  • 00:54Lab-Scale Malachite Green Assay
  • 04:30High-Throughput Screening of Hsp90 Inhibitors by the Malachite Green Assay: Assay Design and Addition Orde
  • 06:03Results: Reactions of Malachite Green Reagent, Standard Plot for Standard Phosphate Concentration, and IC50 Calculation for Geldanamycin Against Yeast Hsp90
  • 07:26Conclusion

Summary

자동 번역

자동 번역

The malachite green assay protocol is a simple and cost-effective method to discover heat shock protein 90 (Hsp90) suppressors, as well as other inhibitor compounds against ATP-dependent enzymes.

Tags

Malachite Green AssayHsp90ATPase ActivityPhosphate DetectionATP HydrolysisInhibitorsHigh-throughput ScreeningGeldanamycinSerial DilutionBlankPositive ControlAbsorbance

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