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Application of AlDeSense to Stratify Ovarian Cancer Cells Based on Aldehyde Dehydrogenase 1A1 Activity
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Application of AlDeSense to Stratify Ovarian Cancer Cells Based on Aldehyde Dehydrogenase 1A1 Activity

Application of AlDeSense to Stratify Ovarian Cancer Cells Based on Aldehyde Dehydrogenase 1A1 Activity

DOI:
10.3791/64713-v

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09:59 min

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March 31, 2023

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, , ,
1Department of Chemistry,University of Illinois Urbana-Champaign, 2Department of Biochemistry,University of Illinois Urbana-Champaign, 3Beckman Institute for Advanced Science and Technology,University of Illinois Urbana-Champaign, 4Cancer Center of Illinois,University of Illinois Urbana-Champaign

Chapters

  • 00:04Introduction
  • 00:38Fluorescence Microscopy Imaging of Cells with High ALDH1A1 Activity
  • 03:03Application of Flow Cytometry to Identify Cells with High ALDH1A1 Activity
  • 07:20Results: Total ALDH1A1 Activity and Identifying the Population of ALDH1A1+ Cells
  • 09:33Conclusion

Summary

자동 번역

자동 번역

Methods to measure ALDH1A1 activity in live cells are critical in cancer research due to its status as a biomarker of stemness. In this study, we employed an isoform-selective fluorogenic probe to determine the relative levels of ALDH1A1 activity in a panel of five ovarian cancer cell lines.

Tags

ALDH1A1Stem Cell BiomarkerAlDeSenseFlow CytometryLive Cell ImagingFluorogenic ProbeConfocal MicroscopyCell CultureCell CountingCell DetachmentTrypsinImage Analysis

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