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Immunology and Infection

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JoVE Journal Immunology and Infection
Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells
 

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Article DOI: 10.3791/64918-v • 10:20 min • March 24th, 2023
March 24th, 2023 •
1, 1, 1,2,3,4, 1, 2, 1
1Quantitative Immuno-hematology, CNRS UMR168, Institut Curie, 2Hamilton Institute, Maynooth University, 3Institut Curie, PSL Research University, CNRS UMR 3348, Orsay, 4Université Paris-Sud, Université Paris-Saclay, CNRS UMR 3348, Orsay

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요약
Automatic Translation

Presented here is a flow cytometry-based technique that allows for simultaneously measuring the number of cell divisions, surface cell phenotype, and cellular kinship. Those properties can be tested statistically using a permutation-based framework.

Tags

Flow Cytometry Hematopoietic Stem Cells Progenitor Cells Kinship Assessment Division Number Assessment Phenotype Assessment Cell Fate Cell Commitment Single Cell Level High Throughput Complex Manipulation Cancer Biology Immunology Developmental Biology CD34+ Fraction CD34- Fraction CFSE High Solution CFSE Low Solution Dulbecco's Modified Eagle Medium (DMEM) Fetal Bovine Serum (FBS) Cell Dye
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