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体内神经元钙处理的亚细胞成像

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Subcellular Imaging of Neuronal Calcium Handling In Vivo

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体内神经元钙处理的亚细胞成像

DOI:

10.3791/64928-v

•

07:14 min

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March 17, 2023

•

Rachel Doser*¹, Kaz M. Knight*^1,2, Ennis Deihl, Frederic Hoerndli

¹Department of Biomedical Sciences,Colorado State University College of Veterinary Medicine and Biomedical Sciences, ²Cellular and Molecular Biology Graduate Program,Colorado State University College of Veterinary Medicine and Biomedical Sciences

Chapters

00:04Introduction
00:51Creating Transgenic Strains
01:47Optical Setup
02:21Preparation of Worms for Imaging
04:14Acquisition of High‐Resolution Image Streams
04:52Results: Rapid Imaging of Calcium Uptake in AVA Neurite
06:19Conclusion

Summary

자동 번역

目前的方法描述了一种非比率方法，使用现成的遗传编码钙指示剂在秀丽隐杆线虫中进行高分辨率、亚区室钙成像。

Tags

Subcellular Imaging Neuronal Calcium Handling In Vivo C. Elegans Calcium Indicator Transgenic Strains Time-lapse Imaging Agar Pads Fluorescent Tools Calcium Signaling Neurodegeneration

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