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1Department of Neurosurgery,Johns Hopkins University School of Medicine, 2Department of Biomedical Engineering,Johns Hopkins University, 3HEPIUS Innovation Laboratory,Johns Hopkins University School of Medicine, 4Department of Electrical Engineering and Computer Science,Johns Hopkins University, 5Department of Mechanical Engineering,Johns Hopkins University, 6Department of Anesthesiology and Critical Care Medicine,Johns Hopkins University
Chapters
- 00:04Introduction
- 01:11Low‐Intensity Focused Ultrasound Assembly and Setup
- 02:20Animal Preparation and Surgical Laminectomy
- 03:38Target Localization Using Laser Guidance
- 04:22Microbubble Administration
- 05:27Spinal Cord Extraction, Tissue Processing, and Visualization of BSCB Disruption
- 06:34Results: Confirmation of Target Localization and Evaluating BSCB Disruption by EBD Extravasation into the Spinal Cord
- 08:17Conclusion
Kan-omurilik bariyerinin (BSCB) bozulması, mikrokabarcıkların intravenöz uygulaması ve düşük yoğunluklu odaklanmış ultrason (LIFU) uygulaması ile başarılı bir şekilde sağlanabilir. Bu protokol, ekipman kurulumu, mikro kabarcık enjeksiyonu, hedef lokalizasyonu ve BSCB bozulma görselleştirmesi dahil olmak üzere bir kemirgen modelinde LIFU kullanılarak BSCB'nin açılmasını detaylandırır.
