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Somatic Genom-Engineered Mausmodelle mit In-vivo-Mikroinjektion und Elektroporation
JoVE 신문
암 연구학
This content is Free Access.
JoVE 신문 암 연구학
Somatic Genome-Engineered Mouse Models Using In Vivo Microinjection and Electroporation
DOI:

08:06 min

May 05, 2023

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Chapters

  • 00:05Introduction
  • 01:01Preparing Microinjection Needle, Surgery Area, Solution, and Needle for Injection
  • 02:26Exposing the Female Reproductive Tract
  • 04:11In Vivo Oviduct Injection and Electroporation
  • 06:29Results: Validation of Successful In Vivo Injection and Electroporation of Oviduct Epithelial Cells
  • 07:16Conclusion

Summary

자동 번역

Dieses Protokoll beschreibt die Mikroinjektion und In-vivo-Elektroporation für die regional eingeschränkte CRISPR-vermittelte Genom-Editierung im Eileiter der Maus.

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