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基因枪子弹的制备和神经元的基因枪转染切片文化
JoVE 신문
생물학
This content is Free Access.
JoVE 신문 생물학
Preparation of Gene Gun Bullets and Biolistic Transfection of Neurons in Slice Culture
DOI:

17:42 min

February 13, 2008

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Chapters

  • 00:02Title
  • 01:02Introduction
  • 01:51Preparing DNA-Coated Gold Beads
  • 05:36Coating Plastic Tubing
  • 08:51Cutting the Plastic Tubing
  • 10:18Shooting Tissue Slices
  • 14:20Results
  • 15:53Troubleshooting
  • 17:04Conclusion

Summary

자동 번역

我们描述了一个准备DNA涂金子弹的方法,并展示了使用这种子弹biolistically转染培养的海马神经元。

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