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Actine Co-Sedimentatie Assay, want de analyse van eiwit binding aan F-actine
JoVE 신문
생물학
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JoVE 신문 생물학
Actin Co-Sedimentation Assay; for the Analysis of Protein Binding to F-Actin
DOI:

07:53 min

March 28, 2008

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Chapters

  • 00:00Title
  • 00:42Introduction
  • 01:44Preparation of Actin Filaments
  • 02:49Preparation of Protein and Binding of Actin
  • 04:26Running the Gel
  • 06:58Conclusion

Summary

자동 번역

Eiwitten binden aan filamenteuze actine (F-actine) door middel van verschillende actine-bindend modules. In deze video laten we zien de procedure van de actine co-sedimentatie, die een in vitro test routinematig gebruikt om eiwitten of specifieke domeinen die binden F-actine te analyseren.

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