Actin Co-Sedimentation Assay; for the Analysis of Protein Binding to F-Actin

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Actin Co-Sedimentation Assay; for the Analysis of Protein Binding to F-Actin

DOI:

10.3791/690-v

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07:53 min

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March 28, 2008

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Jyoti Srivastava, Diane Barber

¹Department of Cell and Tissue Biology,University of California, San Francisco – UCSF

Chapters

00:00Title
00:42Introduction
01:44Preparation of Actin Filaments
02:49Preparation of Protein and Binding of Actin
04:26Running the Gel
06:58Conclusion

Summary

자동 번역

Proteins bind to filamentous actin (F-actin) through distinct actin binding modules. In this video we demonstrate the procedure of actin co-sedimentation, which is an in vitro assay routinely used to analyze proteins or specific domains that bind F-actin.

Actin Co-Sedimentation Assay; for the Analysis of Protein Binding to F-Actin

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Summary

자동 번역

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