John F. X. Diffley Chromosome Replication Laboratory Francis Crick Institute Biography Publications Institution JoVE Articles John F. X. Diffley has not added a biography. If you are John F. X. Diffley and would like to personalize this page please email our Author Liaison for assistance. Publications Unwinding of a Eukaryotic Origin of Replication Visualized by Cryo-EM Nature Structural & Molecular Biology. May, 2024 | Pubmed ID: 38760633 A Chromatinized Origin Reduces the Mobility of ORC and MCM Through Interactions and Spatial Constraint Nature Communications. Oct, 2023 | Pubmed ID: 37872142 Nucleotide Binding Halts Diffusion of the Eukaryotic Replicative Helicase During Activation Nature Communications. Apr, 2023 | Pubmed ID: 37059705 A Replication Fork Determinant for the Establishment of Sister Chromatid Cohesion Cell. Feb, 2023 | Pubmed ID: 36693376 Cyclin E-induced Replicative Stress Drives P53-dependent Whole-genome Duplication Cell. Feb, 2023 | Pubmed ID: 36681079 A DNA Replication Fork-centric View of the Budding Yeast DNA Damage Response DNA Repair. Nov, 2022 | Pubmed ID: 36108423 Mechanism of Replication Origin Melting Nucleated by CMG Helicase Assembly Nature. Jun, 2022 | Pubmed ID: 35705812 The Initiation of Eukaryotic DNA Replication Annual Review of Biochemistry. Jun, 2022 | Pubmed ID: 35320688 Structural Mechanism for the Selective Phosphorylation of DNA-loaded MCM Double Hexamers by the Dbf4-dependent Kinase Nature Structural & Molecular Biology. Jan, 2022 | Pubmed ID: 34963704 Eukaryotic DNA Replication with Purified Budding Yeast Proteins Methods in Enzymology. 2021 | Pubmed ID: 34776208 Identifying SARS-CoV-2 Antiviral Compounds by Screening for Small Molecule Inhibitors of Nsp14 RNA Cap Methyltransferase The Biochemical Journal. Jul, 2021 | Pubmed ID: 34198328 Identifying SARS-CoV-2 Antiviral Compounds by Screening for Small Molecule Inhibitors of Nsp5 Main Protease The Biochemical Journal. Jul, 2021 | Pubmed ID: 34198327 Identifying SARS-CoV-2 Antiviral Compounds by Screening for Small Molecule Inhibitors of Nsp14/nsp10 Exoribonuclease The Biochemical Journal. Jul, 2021 | Pubmed ID: 34198326 Identifying SARS-CoV-2 Antiviral Compounds by Screening for Small Molecule Inhibitors of Nsp3 Papain-like Protease The Biochemical Journal. Jul, 2021 | Pubmed ID: 34198325 Identifying SARS-CoV-2 Antiviral Compounds by Screening for Small Molecule Inhibitors of Nsp15 Endoribonuclease The Biochemical Journal. Jul, 2021 | Pubmed ID: 34198324 Identifying SARS-CoV-2 Antiviral Compounds by Screening for Small Molecule Inhibitors of Nsp12/7/8 RNA-dependent RNA Polymerase The Biochemical Journal. Jul, 2021 | Pubmed ID: 34198323 Identifying SARS-CoV-2 Antiviral Compounds by Screening for Small Molecule Inhibitors of Nsp13 Helicase The Biochemical Journal. Jul, 2021 | Pubmed ID: 34198322 Author's Overview: Identifying SARS-CoV-2 Antiviral Compounds The Biochemical Journal. Jul, 2021 | Pubmed ID: 34198320 An Improved Method for the Incorporation of Fluoromethyl Ketones into Solid Phase Peptide Synthesis Techniques RSC Advances. Jun, 2021 | Pubmed ID: 34178310 Unchecked Nick Ligation Can Promote Localized Genome Re-replication Current Biology : CB. Jun, 2021 | Pubmed ID: 34102115 Budding Yeast Rap1, but Not Telomeric DNA, is Inhibitory for Multiple Stages of DNA Replication in Vitro Nucleic Acids Research. Jun, 2021 | Pubmed ID: 34048583 DNA Replication Origins Retain Mobile Licensing Proteins Nature Communications. Mar, 2021 | Pubmed ID: 33772005 Multiplex Cell Fate Tracking by Flow Cytometry Methods and Protocols. Jul, 2020 | Pubmed ID: 32709120 Mechanism of Head-to-head MCM Double-hexamer Formation Revealed by Cryo-EM Nature. Nov, 2019 | Pubmed ID: 31748745 Rpd3L Contributes to the DNA Damage Sensitivity of Checkpoint Mutants Genetics. Feb, 2019 | Pubmed ID: 30559326 Structure of DNA-CMG-Pol Epsilon Elucidates the Roles of the Non-catalytic Polymerase Modules in the Eukaryotic Replisome Nature Communications. Nov, 2018 | Pubmed ID: 30498216 The Mechanism of Eukaryotic CMG Helicase Activation Nature. Mar, 2018 | Pubmed ID: 29489749 Cryo-EM Structure of a Licensed DNA Replication Origin Nature Communications. Dec, 2017 | Pubmed ID: 29269875 Bidirectional Eukaryotic DNA Replication is Established by Quasi-symmetrical Helicase Loading Science (New York, N.Y.). Jul, 2017 | Pubmed ID: 28729513 Cdt1 Stabilizes an Open MCM Ring for Helicase Loading Nature Communications. Jun, 2017 | Pubmed ID: 28643783 CMG-Pol Epsilon Dynamics Suggests a Mechanism for the Establishment of Leading-strand Synthesis in the Eukaryotic Replisome Proceedings of the National Academy of Sciences of the United States of America. Apr, 2017 | Pubmed ID: 28373564 How the Eukaryotic Replisome Achieves Rapid and Efficient DNA Replication Molecular Cell. Jan, 2017 | Pubmed ID: 27989442 Chromatin Controls DNA Replication Origin Selection, Lagging-Strand Synthesis, and Replication Fork Rates Molecular Cell. Jan, 2017 | Pubmed ID: 27989438 Phosphopeptide Binding by Sld3 Links Dbf4-dependent Kinase to MCM Replicative Helicase Activation The EMBO Journal. May, 2016 | Pubmed ID: 26912723 MCM: One Ring to Rule Them All Current Opinion in Structural Biology. Apr, 2016 | Pubmed ID: 26866665 On the Road to Replication EMBO Molecular Medicine. Feb, 2016 | Pubmed ID: 26787652 Recruitment of Mcm10 to Sites of Replication Initiation Requires Direct Binding to the Minichromosome Maintenance (MCM) Complex The Journal of Biological Chemistry. Mar, 2016 | Pubmed ID: 26719337 Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG Daniel Ramírez Montero1, Humberto Sánchez1, Edo van Veen1, Theo van Laar1, Belén Solano1, John F. X. Diffley2, Nynke H. Dekker1,3 1Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, 2Chromosome Replication Laboratory, Francis Crick Institute, 3Department of Physics and Kavli Institute of Nanoscience Discovery, University of Oxford JoVE 67076 Bioquímica
Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG Daniel Ramírez Montero1, Humberto Sánchez1, Edo van Veen1, Theo van Laar1, Belén Solano1, John F. X. Diffley2, Nynke H. Dekker1,3 1Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, 2Chromosome Replication Laboratory, Francis Crick Institute, 3Department of Physics and Kavli Institute of Nanoscience Discovery, University of Oxford JoVE 67076 Bioquímica
