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Crude Vector Production and Efficient DNA Delivery in Cell Cultures

October 20th, 2023
1, 1,2, 1, 1, 1
1Department of Molecular and Cell Biology, University of California, Berkeley, 2Biophysics Graduate Group, University of California, Berkeley

Summary
Automatic Translation

Rekombinant adeno-associeret virus (rAAV) anvendes i vid udstrækning til klinisk og præklinisk genlevering. En undervurderet anvendelse til rAAV'er er den robuste transduktion af dyrkede celler uden behov for oprensning. For forskere, der er nye inden for rAAV, leverer vi en protokol til transgen-kassettekloning, råvektorproduktion og transduktion af cellekultur.

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