A Comprehensive Review of Avian Embryonic Skin Explant Culture Protocols

September 15, 2023

Using Avian Skin Explants to Study Tissue Patterning and Organogenesis

02:28 min

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Chicken Skin Explant Culture for Organogenesis Study

02:15 min

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Chicken Skin Epithelial-Mesenchymal Recombination Culture

02:17 min

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Tissue Studies by Culturing Reconstituted Chicken Skin

02:39 min

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Tingxin Jiang, Maeve Secor, Rusty Lansford, Randall B. Widelitz, Cheng Ming Chuong

¹Department of Pathology,Keck School of Medicine of University of Southern California, ²Molecular and Computational Biology,University of Southern California, ³Department of Radiology,Keck School of Medicine of University of Southern California

Summary

Here we describe protocols for three types of avian embryonic skin explant cultures that can be used to examine tissue interactions, 4D imaging timelapse movie (3D plus time), global or local perturbation of molecular function, and systems biology characterization.