Comparison of Two Representative Methods for Differentiation of Human Induced Pluripotent Stem Cells into Mesenchymal Stromal Cells

Fangqin Wang; Langping Gao; Xudong Fu; Qintao Yan; Lidan Hu; Jianhua Mao

doi:10.3791/65729

Characterizing Surface Antigens and Proliferation Ability in hiPSC-Driving MSCs

October 20, 2023

Sammenligning av to representative metoder for differensiering av humane induserte pluripotente stamceller til mesenkymale stromale celler

01:06 min

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MSCs differensiering fra hiPSCs via EB-dannelse og monolagskultur

05:21 min

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Fangqin Wang, Langping Gao³, Xudong Fu, Qintao Yan, Lidan Hu², Jianhua Mao³

¹National Clinical Research Center for Child Health,The Children’s Hospital, Zhejiang University School of Medicine, ²Department of Nephrology,The Children’s Hospital, Zhejiang University School of Medicine, ³Zhejiang University School of Medicine, ⁴Liangzhu Laboratory,Zhejiang University

Summary

Denne protokollen beskriver og sammenligner to representative metoder for å differensiere hiscc til mesenkymale stromale celler (MSC). Monolagsmetoden er preget av lavere kostnad, enklere drift og enklere osteogen differensiering. Embryoidlegememetoden (EB) er preget av lavere tidsforbruk.

Characterizing Surface Antigens and Proliferation Ability in hiPSC-Driving MSCs

Sammenligning av to representative metoder for differensiering av humane induserte pluripotente stamceller til mesenkymale stromale celler

MSCs differensiering fra hiPSCs via EB-dannelse og monolagskultur

Summary

Article