Transendothelial Resistance Measurement to Assess Cell Barrier Integrity

September 29, 2023

通过跨内皮电阻检测对bEnd.3血管内皮细胞进行屏障功能完整性记录

bEnd.3 细胞的培养和 CoCl₂ 处理后的细胞活力

用于测量TEER的体外血脑屏障模型组装

体外血脑屏障模型中TEER测量后的屏障破坏和统计分析

Fuhan Fan, Hong Jiang, Ya Hou, Yi Zhang^2,3, Qipeng Zhao, Yong Zeng², Xianli Meng^2,5, Xiaobo Wang

¹School of Pharmacy/School of Modern Chinese Medicine Industry,Chengdu University of Traditional Chinese Medicine, ²Meishan Hospital of Chengdu University of Traditional Chinese Medicine, ³School of Ethnic Medicine,Chengdu University of Traditional Chinese Medicine, ⁴School of Pharmacy,Ningxia Medical University, ⁵Innovative Institute of Chinese Medicine and Pharmacy/Academy for Interdiscipline,Chengdu University of Traditional Chinese Medicine

Summary

该协议描述了一种可靠且有效的脑血屏障体外模型。该方法使用小鼠脑血管内皮细胞bEnd.3并测量跨膜电阻。

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