AAV Isolation by Double Iodixanol Density Gradient Centrifugation

Summary

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Adeno-associated virus is produced in suspension cell culture and purified by double iodixanol density gradient centrifugation. Steps are included to increase total virus yield, decrease the risk of virus precipitation, and further concentrate the final virus product. Expected final titers reach 10¹² viral particles/mL and are suitable for pre-clinical in vivo use.