Collaborative Innovation Center of Quantum Matter 1 article published in JoVE Chemistry Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy Jing Guo1, Sifan You1, Zhichang Wang1, Jinbo Peng1, Runze Ma1, Ying Jiang1,2 1International Center for Quantum Materials, School of Physics, Peking University, 2Collaborative Innovation Center of Quantum Matter Here, we present a protocol to investigate the structure and dynamics of interfacial water at the atomic scale, in terms of submolecular resolution imaging, molecular manipulation, and single-bond vibrational spectroscopy.