First Affiliated Hospital of Xi'an Jiaotong University 3 articles published in JoVE Bioengineering Fabrication of Decellularized Spleen Matrix Derived from Rats Lifei Yang1, Yerong Qian2, Aihua Shi1, Shasha Wei1, Xin Liu4, Yi Lv1,2,3, Junxi Xiang2, Peng Liu3 1Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, 3Department of Graduate School, Xi'an Medical University The decellularized spleen matrix (DSM) holds promising applications in the field of liver tissue engineering. This protocol outlines the procedure for preparing rat DSM, which includes harvesting rat spleens, decellularizing them through perfusion, and evaluating the resulting DSM to confirm its characteristics. Cancer Research Characterization and Functional Prediction of Bacteria in Ovarian Tissues Lanbo Zhao*1, Weichu Zhao*2, Qi Wang*1, Dongxin Liang1, Yu Liu3, Guoxing Fu4, Lu Han1, Yiran Wang1, Chao Sun1, Qing Wang1, Qing Song1, Qiling Li1, Qinrui Lu1 1Department of Pathology, First Affiliated Hospital of Xi’an Jiaotong University, 4Omega Bioservices Inc Immunohistochemistry staining and 16S ribosomal RNA gene (16S rRNA gene) sequencing were performed in order to discover and distinguish bacteria in cancerous and noncancerous ovarian tissues in situ. The compositional and functional differences of the bacteria were predicted by using BugBase and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt). Medicine A Training and Testing System for Performing Vascular Reconstruction In Vitro Yue Wang1,2, Lijun Mu3, Wei Zhang1,2, Huan Chen1,2, Qingshan Li1,2, Aihua Shi1, Bo Tang2, Xiaogang Zhang1,2, Dinghui Dong1,2, Yi Lv1,2 1 Here we present a training and testing system where a trainee can complete manual vascular reconstruction in vitro individually using a magnetic anchoring technique. The system can also be used to test the quality of reconstruction.
