Agricultural Research Organization (Volcani Center) 1 article published in JoVE Bioengineering Biomimetic Replication of Root Surface Microstructure using Alteration of Soft Lithography Pallavi Kumari1, Tali Sayas1, Maya Kleiman1,2 1Institute of Plant Sciences, Agricultural Research Organization (Volcani Center), 2Agro-NanoTechnology and Advanced Materials Center, Agricultural Research Organization (Volcani Center) Biomimetics has been previously used as a tool to study leaf-microorganism interactions. However, no such tool exists for roots. Here, we develop a protocol to form synthetic surfaces mimicking root surface microstructure for the study of root-environment interactions.