SASTRA University 1 article published in JoVE Biochemistry RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs Varsha Tirumalai1,2, Melvin Prasad1, P. V. Shivaprasad1 1National Centre for Biological Sciences, Tata Institute of Fundamental Research, 2SASTRA University This method demonstrates use of the northern hybridization technique to detect miRNAs from total RNA extract.