ACECR 1 article published in JoVE Neuroscience Isolation and Culture of Embryonic Mouse Neural Stem Cells Farshad Homayouni Moghadam1, Maryam Sadeghi-Zadeh1,3, Bahareh Alizadeh-Shoorjestan1,3, Reza Dehghani-Varnamkhasti1,3, Sepideh Narimani1,2, Leila Darabi1,3, Abbas Kiani Esfahani1, Mohammad Hossein Nasr Esfahani1 1Department of Cellular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, 2Department of Biology, Faculty of Basic Sciences, Islamic Azad University, 3Department of Biology, ACECR Institute of Higher Education Here, we introduce a novel microsurgical technique for the isolation of neural stem cells from the E13 mouse embryo ganglionic eminence.