Kanazawa University 8 articles published in JoVE Neuroscience In Vivo Targeting of Neural Progenitor Cells in Ferret Neocortex by In Utero Electroporation Nereo Kalebic1,2, Barbara Langen1,3, Jussi Helppi1, Hiroshi Kawasaki4, Wieland B. Huttner1 1Max Planck Institute of Molecular Cell Biology and Genetics, 2Human Technopole, 3Landesdirektion Sachsen, 4Department of Medical Neuroscience, Graduate School of Medical Sciences, Kanazawa University Presented here is a protocol to perform genetic manipulation in the embryonic ferret brain using in utero electroporation. This method allows for targeting of neural progenitor cells in the neocortex in vivo. Behavior Investigation into Deep Breathing through Measurement of Ventilatory Parameters and Observation of Breathing Patterns Masami Yokogawa1, Tomoyo Kurebayashi2, Kazuki Soma3, Hiroichi Miaki1, Takao Nakagawa4 1Faculty of Health Sciences, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, 2Rehabilitation Section, Higashimatsuyama Municipal Hospital, 3Division of Health Sciences, Graduate School of Medical Sciences, Kanazawa University, 4Department of Physical and Rehabilitation Medicine, Kanazawa Medical University Here, we present a protocol to assess two deep breathing patterns of natural and diaphragmatic breathing for their effectiveness and ease of execution. Fifteen participants were selected, utilizing an electrocardiograph and expired gas analyzer for measurement of the ventilatory parameters, together with visual assessment by video capture of thoracoabdominal movement. Neuroscience Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice Shota Kodani*1, Shingo Soya*2, Takeshi Sakurai2,3 1Department of Molecular Neuroscience and Integrative Physiology, Faculty of Medicine, Kanazawa University, 2International Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba, 3Faculty of Medicine, University of Tsukuba Here, we describe methods of optogenetic manipulation of particular types of neurons during monitoring of sleep/wakefulness states in mice, presenting our recent work on the bed nucleus of the stria terminalis as an example. Neuroscience Visualization of Thalamocortical Axon Branching and Synapse Formation in Organotypic Cocultures Naoyuki Matsumoto1,2, Nobuhiko Yamamoto2 1Department of Medical Neuroscience, Graduate School of Medical Sciences, Kanazawa University, 2Neuroscience Laboratories, Graduate School of Frontier Biosciences, Osaka University This protocol describes a method for simultaneous imaging of thalamocortical axon branching and synapse formation in organotypic cocultures of the thalamus and cerebral cortex. Individual thalamocortical axons and their presynaptic terminals are visualized by a single cell electroporation technique with DsRed and GFP-tagged synaptophysin. Medicine A Novel Method: Super-selective Adrenal Venous Sampling Kohzoh Makita1, Koshiro Nishimoto2, Kanako Kiriyama-Kitamoto3, Shigehiro Karashima4, Tsugio Seki5, Masanori Yasuda6, Seishi Matsui7, Masao Omura3, Tetsuo Nishikawa3 1Department of Radiology, Nerima Hikarigaoka Hospital, 2Department of Uro-Oncology, Saitama Medical University International Medical Center, 3Endocrinology & Diabetes Center, Yokohama Rosai Hospital, 4Division of Endocrinology and Metabolism, Kanazawa University Graduate School of Medicine, 5Department of Medical Education, School of Medicine, California University of Science and Medicine, 6Department of Pathology, Saitama Medical University International Medical Center, 7Department of Radiology, Yokohama Rosai Hospital 'Super-selective' adrenal venous sampling (ssAVS, also called segmental adrenal venous sampling: sAVS) was performed using a micro-catheter to identify adrenal segment(s) that produce excessive amounts of hormones. The ssAVS technique was described, cases in which adrenal segmental lesion(s) were identified by ssAVS were presented, and the usefulness of ssAVS in future adrenal research was discussed. Immunology and Infection Phagocytosis Assay for Apoptotic Cells in Drosophila Embryos Saori Nonaka1, Aki Hori1, Yoshinobu Nakanishi1, Takayuki Kuraishi1 1Graduate School of Medical Sciences, Kanazawa University We herein describe a phagocytosis assay using the dispersed embryonic cells of Drosophila. It enables us to easily and precisely quantify in vivo phagocytosis levels, and to identify new molecules required for the phagocytosis of apoptotic cells. Medicine A Detailed Protocol for Perspiration Monitoring Using a Novel, Small, Wireless Device Kazuhiro Ogai1, Masakazu Fukuoka2, Kei-ichiro Kitamura3, Kiyoshi Uchide4, Tetsu Nemoto3 1Wellness Promotion Science Center, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, 2Advanced Research Center for Human Sciences, Waseda University, 3Department of Clinical Laboratory Science, Graduate School of Medical Science, Kanazawa University, 4Asanogawa General Hospital Recently, we developed a small wireless device for perspiration monitoring. In this article, we present detailed protocols on how to use the device for perspiration monitoring with an example of the sympathetic activity test. Biology Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1 Karthika Natarajan1,2, Yi Xie1,3, Takeo Nakanishi4, Rebecca S. Moreci5,6, Pancharatnam Jeyasuria7, Arif Hussain1,3,8,9, Douglas D. Ross1,3,8,9,10,11 1Greenebaum Cancer Center, University of Maryland School of Medicine, 2Pharmaceutical Sciences, University of Maryland School of Pharmacy, 3Baltimore VA Medical Center, 4Membrane Transport and Biopharmaceutics, School of Pharmaceutical Sciences, Kanazawa University, 5Obstetrics, Gynecology and Reproductive Science, University of Pittsburgh, 6 With the murine ABC transporter Bcrp1 (Abcg2) as an example, in-silico protocols are presented to detect alternative promoter usage in genes expressed in mouse tissues, and to evaluate the functionality of the alternative promoters identified using reporter assays.