Toyo University 2 articles published in JoVE Environment Quantification of Endogenous Auxin and Cytokinin During Internode Culture of Ipecac Imari Koike1, Koichiro Shimomura1, Mikihisa Umehara1 1Graduate School of Life Sciences, Toyo University Adventitious shoots can be induced on internodal segments of ipecac without phytohormone treatment. To evaluate phytohormone dynamics during adventitious shoot formation, we measured endogenous auxin and cytokinin in internodal segments by LC-MS/MS. Developmental Biology Salinity-dependent Toxicity Assay of Silver Nanocolloids Using Medaka Eggs Chisato Kataoka1,2, Shosaku Kashiwada1,2 1Graduate School of Life Sciences, Toyo University, 2Research Center for Life and Environmental Sciences, Toyo University Embryonic stages are the most susceptible to xenobiotics. Although chemical toxicity depends on salinity, no method exists to test the salinity dependence of toxicity to aquatic organisms. Here, we describe a new and high-throughput method for determining the salinity dependence of toxicity to aquatic embryos.