Nihon Pharmaceutical University 2 articles published in JoVE Biochemistry The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes Hiroyuki Yamamoto1, Yoshikazu Sawaguchi2, Michio Kimura1 1Department of Microbiology and Molecular Cell Biology, Nihon Pharmaceutical University, 2Department of Clinical Pharmaceutics, Nihon Pharmaceutical University Here, we present a protocol to determine protease specificity in crude mouse tissue extracts using MALDI-TOF spectrometry. Environment Protocol for Measuring the Thermal Properties of a Supercooled Synthetic Sand-water-gas-methane Hydrate Sample Michihiro Muraoka1, Naoko Susuki1, Hiroko Yamaguchi2, Tomoya Tsuji3, Yoshitaka Yamamoto1 1Research Institute of Energy Frontier, National Institute of Advanced Industrial Science and Technology (AIST), 2College of Industrial Technology, Nihon University, 3SHIZEN ikohza, Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia We present a protocol for measuring the thermal properties of synthetic hydrate-bearing sediment samples comprising sand, water, methane, and methane hydrate.