Hosei University 2 articles published in JoVE Biology Reactivation of Demembranated Cell Models in Chlamydomonas reinhardtii Noriko Ueki1, Atsuko Isu2, Ken‒ichi Wakabayashi2,3 1Science Research Center, Hosei University, 2Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, 3School of Life Science and Technology, Tokyo Institute of Technology The in vitro reactivation of motile cells is a crucial experiment in understanding the mechanisms of cell motility. The protocol describes reactivating the demembranated cell models of Chlamydomonas reinhardtii, a model organism to study cilia/flagella. Biology Observation of Photobehavior in Chlamydomonas reinhardtii Noriko Ueki*1, Atsuko Isu*2, Ayaka Kyuji*2,3, Yuma Asahina*2,3, Satoaki So*2,3, Rina Takahashi*2,3, Toru Hisabori2,3, Ken‒ichi Wakabayashi2,3 1Science Research Center, Hosei University, 2Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, 3School of Life Science and Technology, Tokyo Institute of Technology Most swimming photoautotrophic organisms show photo-induced behavioral changes (photobehavior). The present protocol observes the said photobehavior in the model organism Chlamydomonas reinhardtii.