Hacettepe University 1 article published in JoVE Biochemistry Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B Zekiye Ceren Arituluk1,2, Bishnu Adhikari1, Urmila Maitra1, Caroline Goodman1, Lukasz M. Ciesla1 1Department of Biological Sciences, The University of Alabama, 2Department of Pharmaceutical Botany, Faculty of Pharmacy, Hacettepe University The protocol describes the preparation of cell membrane affinity chromatography (CMAC) columns with immobilized cell membrane fragments containing functional transmembrane tropomyosin kinase receptor B proteins. The use of CMAC columns in the identification of specialized plant metabolites interacting with these receptors and present in complex natural mixtures is also explained.