Chinese Institute for Brain Research 1 article published in JoVE Neuroscience Calcium Imaging in Mouse Superior Colliculus Zhe Li*1, Ruixiang Wu*1, Ya-tang Li1 1Chinese Institute for Brain Research This protocol details the procedure for imaging calcium responses in the superior colliculus (SC) of awake mice, including imaging single-neuron activity with two-photon microscopy while leaving the cortex intact in wild-type mice, and imaging the entire SC with wide-field microscopy in partial-cortex mutant mice.