Bond University 1 article published in JoVE Developmental Biology Basement Membrane Matrix Encapsulated Cell Aggregation for Investigating Murine Spleen Tissue Formation Karin Tourle1, Amber Rucinski1, Alexander Grainger1, Ioannis J. Limnios1, Macarena Gonzalez Ruiz1, Jonathan K.H. Tan1 1Clem Jones Centre for Regenerative Medicine, Faculty of Health Sciences and Medicine, Bond University This article describes a protocol for aggregating and encapsulating spleen cells within a semi-solid basement membrane matrix. Basement membrane matrix constructs can be used in three-dimensional culture for studying organoid development, or for in vivo transplantation and tissue regeneration studies.