Max Planck Institute of Colloids and Interfaces 2 articles published in JoVE Chemistry Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy Bastian Kubsch1, Tom Robinson1, Jan Steinkühler1, Rumiana Dimova1 1Max Planck Institute of Colloids and Interfaces Experiments on phase separated giant unilamellar vesicles (GUVs) frequently neglect physiological solution conditions. This work presents approaches to study the effect of high-salinity buffer on liquid-liquid phase separation in charged multicomponent GUVs as a function of trans-membrane solution asymmetry and temperature. Chemistry Preparation of Carbon Nanosheets at Room Temperature Stephen Schrettl1, Bjoern Schulte1, Cristina Stefaniu2, Joana Oliveira2, Gerald Brezesinski2, Holger Frauenrath1 1Institute of Materials, Ecole Polytechnique Fédérale de Lausanne (EPFL), 2Colloid Chemistry Department, Max Planck Institute of Colloids and Interfaces We present the synthesis of an amphiphilic hexayne and its use in the preparation of carbon nanosheets at the air-water interface from a self-assembled monolayer of these reactive, carbon-rich molecular precursors.