University Hospital Regensburg 4 articles published in JoVE Bioengineering Demonstration of Self-Assembled Cell Sheet Culture and Manual Generation of a 3D Tendon/Ligament-Like Organoid by using Human Dermal Fibroblasts Ana Luísa Graça1, Niklas Kroner-Weigl2, Viviana Reyes Alcaraz1, Sigrid Müller-Deubert1, Maximilian Rudert3, Denitsa Docheva1 1Department of Musculoskeletal Tissue Regeneration, Orthopaedic Hospital König-Ludwig-Haus, University of Würzburg, 2Laboratory for Experimental Trauma Surgery, Department of Trauma Surgery, University Hospital Regensburg, 3Department of Orthopaedics, Orthopaedic Hospital König-Ludwig-Haus, University of Würzburg Herein, we demonstrate a three-step organoid model (two-dimensional [2D] expansion, 2D stimulation, three-dimensional [3D] maturation) offering a promising tool for tendon fundamental research and a potential scaffold-free method for tendon tissue engineering. Cancer Research In Vivo Immunogenicity Screening of Tumor-Derived Extracellular Vesicles by Flow Cytometry of Splenic T Cells Florian Stritzke1,2, Hendrik Poeck1,2,3,4, Simon Heidegger1,2 1Department of Medicine III, School of Medicine, Technical University of Munich, 2Center for Translational Cancer Research (TranslaTUM), School of Medicine, Technical University of Munich, 3Department of Internal Medicine III, University Hospital Regensburg, 4National Centre for Tumor Diseases WERA This manuscript describes how to assess in vivo immunogenicity of tumor cell-derived extracellular vesicles (EVs) using flow cytometry. EVs derived from tumors undergoing treatment-induced immunogenic cell death seem particularly relevant in tumor immunosurveillance. This protocol exemplifies the assessment of oxaliplatin-induced immunostimulatory tumor EVs but can be adapted to various settings. Neuroscience Brain Death Induction in Mice Using Intra-Arterial Blood Pressure Monitoring and Ventilation via Tracheostomy Paul V. Ritschl*1,2,3, Lena Hofhansel*2,4, Bernhard Flörchinger5, Rupert Oberhuber2, Robert Öllinger1, Johann Pratschke1, Katja Kotsch6 1Department of Surgery Campus Charité Mitte/Campus Virchow-Klinikum, Charité - Universitätsmedizin Berlin, 2Department of Visceral, Transplant and Thoracic Surgery, Medical University of Innsbruck, 3Charité Clinician Scientist Program, Berlin Institute of Health, 4Department of Psychiatry, Psychotherapy and Psychosomatics, RWTH Aachen University, 5Department of Cardiothoracic Surgery, University Medical Center Regensburg, 6Department of General, Visceral and Vascular Surgery, Charité - Universitätsmedizin Berlin We present a murine model of brain death induction in order to evaluate the influence of its pathophysiological effects on organs as well as on consecutive grafts in the context of solid organ transplantation. Medicine The Influence of Liver Resection on Intrahepatic Tumor Growth Hannes H. Brandt1, Valérie Nißler2, Roland S. Croner3 1Friedrich-Alexander-University Erlangen-Nürnberg (FAU), 2Department of Surgery, University Hospital Regensburg, 3Department of Surgery, University Hospital Erlangen A high incidence of tumor recurrence after resection of liver metastases remains an unsolved problem. The illustrated mouse model may be useful to investigate the reasons for such recurrences. It combines a liver resection model with intrahepatic tumor cell injection for the first time.
