German Federal Institute for Risk Assessment (BfR) 4 articles published in JoVE Behavior Training Laboratory Rabbits to Refine Routine Husbandry Procedures Katharina Hohlbaum1, Pia Kahnau1, Jenny Wilzopolski1, Carola Fischer-Tenhagen1 1German Centre for the Protection of Laboratory Animals (Bf3R), German Federal Institute for Risk Assessment Training protocols involving positive reinforcement for routine husbandry procedures in rabbits were developed and proved to be successful, as the rabbits reliably showed the trained behaviors at the weekly general examination. Picking the rabbits up with the transport box was less aversive than the conventional handling technique. Engineering UV-Vis Spectroscopic Characterization of Nanomaterials in Aqueous Media Ana C. Quevedo1, Emily Guggenheim1, Sophie M. Briffa1, Jessica Adams2,3, Stephen Lofts2, Minjeong Kwak4, Tae Geol Lee4, Colin Johnston5, Stephan Wagner6, Timothy R. Holbrook6, Yves U. Hachenberger7, Jutta Tentschert7, Nicholas Davidson1, Eugenia Valsami-Jones1 1School of Geography, Earth and Environmental Sciences, University of Birmingham, 2UK Centre for Ecology and Hydrology, 3Natural England, 4Center for Nanosafety Metrology, Korea Research Institute of Standards and Science (KRISS), 5Department of Materials, University of Oxford, 6Department of Analytical Chemistry, Helmholtz-Centre for Environmental Research, 7Department of Chemical and Product Safety, German Federal Institute for Risk Assessment (BfR) This study presents the benchmarking results for an interlaboratory comparison (ILC) designed to test the standard operating procedure (SOP) developed for gold (Au) colloid dispersions characterized by ultraviolet-visible Spectroscopy (UV-Vis), amongst six partners from the H2020 ACEnano project for sample preparation, measurement, and analysis of the results. Engineering 3D Printing - Evaluating Particle Emissions of a 3D Printing Pen Heike Sigloch1, Frank S. Bierkandt1, Ajay V. Singh1, Ashish K. Gadicherla2, Peter Laux1, Andreas Luch1 1Department of Chemical and Product Safety, German Federal Institute for Risk Assessment (BfR), 2Department of Biological Safety, German Federal Institute for Risk Assessment (BfR) This protocol presents a method to analyze the emission of 3D printing pens. Particle concentration and particle size distribution of the released particle is measured. Released particles are further analyzed with transmission electron microscopy (TEM). Metal content in filaments is quantified by inductively coupled plasma mass spectrometry (ICP-MS). Chemistry Preparation of Nanoparticles for ToF-SIMS and XPS Analysis Francesca Bennet1, Anja Müller1, Jörg Radnik1, Yves Hachenberger2, Harald Jungnickel2, Peter Laux2, Andreas Luch2, Jutta Tentschert2 1Division of Surface Analysis and Interfacial Chemistry, Federal Institute for Material Research and Testing (BAM), 2Department of Chemical and Product Safety, German Federal Institute for Risk Assessment (BfR) A number of different procedures for preparing nanoparticles for surface analysis are presented (drop casting, spin coating, deposition from powders, and cryofixation). We discuss the challenges, opportunities, and possible applications of each method, particularly regarding the changes in the surface properties caused by the different preparation methods.
