Aarhus University Hospital 13 articles published in JoVE Medicine Positron Emission Tomography Using 64-Copper as a Tracer for the Study of Copper-Related Disorders Ditte Emilie Munk1, Frederik Teicher Kirk1, Mikkel Vendelbo2, Karina Vase2, Ole Munk2, Peter Ott1, Thomas Damgaard Sandahl1 1Department of Hepatology and Gastroenterology, Aarhus University Hospital, 2Department of Nuclear Medicine & PET-Center, Aarhus University Hospital The present protocol describes how to perform 64Cu PET/CT and PET/MRI imaging in humans to study copper-related disorders, such as Wilson disease, and the treatment effect on copper metabolism. Cancer Research Simple and Fast Rolling Circle Amplification-Based Detection of Topoisomerase 1 Activity in Crude Biological Samples Josephine Geertsen Keller1,2, Karol Mizielinski3, Kamilla Vandsø Petersen1,2, Magnus Stougaard2,3,4, Birgitta R. Knudsen1,3, Cinzia Tesauro3 1Department of Molecular Biology and Genetics, Aarhus University, 2Department of Clinical Medicine, Aarhus University, 3VPCIR Biosciences ApS, 4Department of Pathology, Aarhus University Hospital A protocol for the sensitive and quantitative detection of topoisomerase 1 activity using the rolling circle enhanced enzyme activity detection assay is described. The method allows detection of topoisomerase 1 activity from purified components or cell/tissue extracts. This protocol has wide-ranging applications in any field involving detection of enzymatic activity. Neuroscience Conventional and Threshold-Tracking Transcranial Magnetic Stimulation Tests for Single-handed Operation Hatice Tankisi1, James Howells2, Bülent Cengiz3, Gintaute Samusyte4, Martin Koltzenburg5, Hugh Bostock6 1Department of Clinical Neurophysiology, Aarhus University Hospital, 2Central Clinical School, Faculty of Medicine and Health, University of Sydney, 3Department of Neurology, Gazi University Faculty of Medicine, 4Department of Neurology, Medical Academy, Lithuanian University of Health Sciences, 5Department of Clinical Neurophysiology, National Hospital for Neurology and Neurosurgery; Department of Clinical and Movement Neurosciences, UCL Queen Square Institute of Neurology, 6Department of Neuromuscular Diseases, UCL Queen Square Institute of Neurology We present a suite of standardized single- and paired-pulse transcranial magnetic stimulation (TMS) recording protocols, with options for conventional amplitude measurements and threshold-tracking. This program can control three different types of magnetic stimulators and is designed to enable all tests to be performed conveniently by a single operator. Medicine Closed Chest Biventricular Pressure-Volume Loop Recordings with Admittance Catheters in a Porcine Model Mads Dam Lyhne1,2, Jacob Gammelgaard Schultz1,2, Simone Juel Dragsbaek1,2, Jacob Valentin Hansen1,2, Christian Schmidt Mortensen1,2, Anders Kramer1,2, Jens Erik Nielsen-Kudsk1,2, Asger Andersen1,2 1Department of Cardiology Research, Aarhus University Hospital, 2Department of Clinical Medicine, Aarhus University Here we present a closed chest approach to admittance-based bi-ventricular pressure-volume loop recordings in pigs with acute right ventricular dysfunction. Neuroscience Muscle Velocity Recovery Cycles to Examine Muscle Membrane Properties Agnes Witt1, Hugh Bostock2, Werner J. Z'Graggen3, S. Veronica Tan4, Alexander Gramm Kristensen1, Rikke Søgaard Kristensen1, Lotte Hardbo Larsen1, Zennia Zeppelin1, Hatice Tankisi1 1Department of Clinical Neurophysiology, Aarhus University Hospital, 2UCL Queen Square Institute of Neurology, Queen Square House, 3Departments of Neurology and Neurosurgery, Inselspital, Bern University Hospital, University of Bern, 4MRC Centre for Neuromuscular Diseases, UCL Institute of Neurology, The National Hospital for Neurology and Neurosurgery Presented here is a protocol for the recording of muscle velocity recovery cycles (MVRCs), a new method of examining muscle membrane properties. MVRCs enable in vivo assessment of muscle membrane potential and alterations in muscle ion channel function in relation to pathology, and it enables the demonstration of muscle depolarization in neurogenic muscles. Immunology and Infection Humanized NOG Mice for Intravaginal HIV Exposure and Treatment of HIV Infection Anna H. F. Andersen1,2, Stine S. F. Nielsen1,2, Rikke Olesen1,2, Katharina Mack1, Frederik Dagnæs-Hansen3, Niels Uldbjerg1,4, Lars Østergaard1,2, Ole S. Søgaard1,2, Paul W. Denton1,2,5, Martin Tolstrup1,2 1Department of Clinical Medicine, Aarhus University, 2Department of Infectious Diseases, Aarhus University Hospital, 3Department of Biomedicine, Aarhus University, 4Department of Gynecology and Obstetrics, Aarhus University Hospital, 5Department of Biology, University of Nebraska at Omaha We have developed a protocol for the generation and evaluation of a humanized and human immunodeficiency virus-infected NOG mouse model based on stem cell transplant, intravaginal human immunodeficiency virus exposure, and droplet digital PCR RNA quantification. Medicine Contusion Spinal Cord Injury via a Microsurgical Laminectomy in the Regenerative Axolotl Mathias Møler Thygesen1,2, Fredrik Guldbæk-Svensson1, Mikkel Mylius Rasmussen2, Henrik Lauridsen1 1Department of Clinical Medicine, Aarhus University, 2Department of Neurosurgery, Aarhus University Hospital This manuscript presents protocols for surgically inflicting controlled blunt and sharp spinal cord injuries to a regenerative axolotl (Ambystoma mexicanum). Medicine A Pulmonary Trunk Banding Model of Pressure Overload Induced Right Ventricular Hypertrophy and Failure Stine Andersen1, Jacob Gammelgaard Schultz1, Sarah Holmboe1, Julie Birkmose Axelsen1, Mona Sahlholdt Hansen1, Mads Dam Lyhne1, Jens Erik Nielsen-Kudsk1, Asger Andersen1 1Department of Clinical Medicine and Cardiology, Aarhus University Hospital We present a surgical method to induce right ventricular hypertrophy and failure in rats. Neuroscience CMAP Scan MUNE (MScan) - A Novel Motor Unit Number Estimation (MUNE) Method Anna B. Jacobsen1, Hugh Bostock2, Hatice Tankisi1 1Department of Clinical Neurophysiology, Aarhus University Hospital, 2Institute of Neurology, Queen Square House This protocol describes a new method to estimate the number of functioning motor units in a muscle, by fitting a model to a detailed stimulus-response curve of the compound muscle action potential. It is quick and easy to perform and analyze and has excellent reproducibility. Neuroscience An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice Nastasia K-H Lim1,2, Visse Moestrup3,4, Xiao Zhang1, Wen-An Wang5, Arne Møller3,4,6, Fu-De Huang1,4 1Shanghai Advanced Research Institute, University of Chinese Academy of Sciences, Chinese Academy of Science, 2Shanghai Institute of Materia Medica, University of Chinese Academy of Sciences, Chinese Academy of Science, 3Center of Functionally Integrative Neuroscience, Department of Clinical Medicine, Aarhus University, 4Sino-Danish Center for Education and Research (SDC), 5Department of Neurology, Xinhua Hospital Chongming Branch Affiliated to Shanghai Jiao Tong University School of Medicine, 6Department of Nuclear Medicine and PET-centre, Aarhus University Hospital This protocol describes an improved technique for the abundant collection of cerebrospinal fluid (CSF) with no contamination from blood. With greater sample collection and purity, more analyses can be performed using CSF to further our understanding of diseases that affect the brain and spinal cord. Developmental Biology Generation and Culturing of Primary Human Keratinocytes from Adult Skin Claus Johansen1 1Department of Dermatology, Aarhus University Hospital The human skin acts as a first line of defense against the external environment. We present a method for isolating primary human keratinocytes from adult skin. These isolated keratinocytes are useful in numerous experimental setups, and are a highly suitable model for studying molecular mechanisms in cutaneous biology in vitro. Neuroscience Exposure of the Pig CNS for Histological Analysis: A Manual for Decapitation, Skull Opening, and Brain Removal Carsten R. Bjarkam1, Dariusz Orlowski2, Laura Tvilling2, Johannes Bech2, Andreas N. Glud2, Jens-Christian H. Sørensen2 1Department of Neurosurgery, Clinical Institute of Medicine, Aalborg University Hospital, 2Center of Experimental Neuroscience (Cense), Department of Neurosurgery, Institute of Clinical Medicine, Aarhus University Hospital The goal of this paper and instructional video is to describe how to expose and remove the postmortem pig brain and pituitary gland in an intact state, suitable for subsequent macroscopic and histological analysis. Medicine A Piglet Model of Neonatal Hypoxic-Ischemic Encephalopathy Kasper J. Kyng1, Torjus Skajaa1, Sigrid Kerrn-Jespersen1, Christer S. Andreassen2, Kristine Bennedsgaard1, Tine B. Henriksen1 1The Perinatal Research Unit, Department of Pediatrics, Institute of Clinical Medicine, Aarhus University Hospital, 2Department of Otorhinolaryngology and Head & Neck Surgery, Institute of Clinical Medicine, Aarhus University Hospital Hypoxic-ischemic encephalopathy following perinatal asphyxia can be studied using animal models. We demonstrate the procedures necessary for establishing a piglet model of neonatal hypoxic-ischemic encephalopathy.